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ELECTRODES

Target sample: xenopus embryo

lf195p0.5_electrode.jpg
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CUY/LF701P2E
CUY = Nepa Gene Co. Part Number Prefix
LF = BEX Co. Part Number Prefix 
= electrode material: platinum coating
= length of square electrode (mm)
= type of electrode E: petridish type L: rod type
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195 Series 
Platinum Needle Electrode

Target sample: adult mouse's brain

lf207_electrodes.jpg
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200S  
 Injection Needle Electrode with Holder

Target sample: single mammalian or plant cell etc.

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Procedure of electroporation with CUY/LF230

  1. Place the tip of a glass capillary on cell membrane

  2. Electroporate a cell

  3. Make a pore in cell membrane and introduce genes into a cell

Advantage of CUY/LF230

  • Able to quantify the amount of DNA introduced into a cell

  • Minimize a damage caused by buffer

Glass capillary and manipulator rod are not included

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lf230_glass_capillary_diagram.jpg
Schematics of CUY/LF230 with Glass Capillary
230 
Glass Capillary Holder with Wire Electrode

Target sample: xenopus embryo

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Schematics of Plate Electrode
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Plate Electrodes for Cultured Adherent Cells

Each plate electrode has a 0.3mm height insulated foot at both ends of the bottom. When an electrode is placed in a dish, as the conductive part of an electrode will not touch cells directly, a foot minimizes damage on cells.

CUY/LF512-5
CUY = Nepa Gene Co. Part Number Prefix
LF = BEX Co. Part Number Prefix 
= no. of plate electrodes
= gap between electrodes (mm)
512_512-14.png
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512 / 513 / 514 Series
Plate Electrode for Cultured Cells

Target sample: mouse or rat's embryo (whole mount culture), extracted organ or tissue

lf520p15_electrode.jpg
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*For whole mount culture

CUY/LF520P5
CUY = Nepa Gene Co. Part Number Prefix
LF = BEX Co. Part Number Prefix 
= electrode material: platinum coating
= gap between electrodes (mm)
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520 Series 
Bath with Plate Electrodes on Petridish

Target sample: mouse and rat's muscle, newborn rat's brain etc.

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Diameter of electrode tip: 0.5mmΦ

CUY/LF560S5
CUY = Nepa Gene Co. Part Number Prefix
LF = BEX Co. Part Number Prefix 
= electrode material: stainless steel
= gap between electrodes (mm)
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560 Series 
 Fixed Gap Needle Electrode
200S  
 Injection Needle Electrode with Holder

Target sample: honey bee's brain, newborn mouse's brain etc.

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567  
Needle Electrode (variable gap)
lf580_electrode_holder.jpg

Electrode gap: 1 - 10mm (1mm increment)
Compatible electrodes: 611 and 613

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 580
Electrode Holder

Target sample: chick embryo's organ or tissue derived from ectoderm (i.e. neural tube)

lf610p4-4_electrode.jpg
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CUY/LF610P2-1
CUY = Nepa Gene Co. Part Number Prefix
LF = BEX Co. Part Number Prefix 
= electrode material: platinum
= gap between electrodes (mm)
1 = length of conductive part (mm)
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610 Series 
Fixed Platinum Needle Electrode (for routine work)

Target sample: chick embryo's organ or tissue derived from ectoderm (i.e. neural tube)

cuytables 2_611.png
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lf611p3-1_electrode.jpg
CUY/LF611P3-1
CUY = Nepa Gene Co. Part Number Prefix
LF = BEX Co. Part Number Prefix 
= electrode material: platinum
= gap between electrodes (mm)
1 = length of conductive part (mm)
611 Series 
Platinum Needle Electrode

Target sample: chick embryo's organ or tissue derived from endoderm and mesoderm

lf613p3_electrode.jpg
cuytables 2_613.png

*Used with 611 or 614 series electrodes

613 Series 
Z-Shape Platinum Electrode*
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